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Quenching Of Fluorescence

Quenching of Fluorescence

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Title: Quenching of Fluorescence

Essay Details:

Subject: Science

Author: Martha G

Date: February 4, 2015

Level: University

Grade: A

Length: 5 / 1387

Essay text:

Emission occurs following an absorption event if the upper state is not relaxed by non-radiative collisional process (called quenching). Dynamic quenching, also called collisional quenching, requires contact between the excited species and the quenching agent (Q). Dynamic quenching occurs as rapidly as the collision partners can diffuse together. The rate is temperature and viscosity dependent. The quencher concentration must be high enough that there is a high probability of collision between the excited species and the quencher during the lifetime of the exited state. When fluorescein is illuminated with light that has a wavelength of 490 nm an electronic transition occurs...

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