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The importance of a plasmid is in the step of cloning and sequencing when the construction of a recombinant DNA molecule occurs. The target gene fragment is ligated to a plasmid, and becomes recombinant DNA. Then the plasmid can replicate autonomously in an appropriate host organism.
II. The polymerase chain reaction (PCR) is the amplification of DNA sequence by repeated cycles of strand separation
and replication. This is a direct method of making copies of a desired DNA sequence, unlike the technique using plasmids. PCR is a process quite like DNA replication. It is still the process of two DNA strands unwinding, replicating, and then reannealing, however the strands are separated by heat...
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