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It is imperative that the tubing doesn't have any bubbles, otherwise the experiment will not work. After it equilibrates for ten minutes, readings are taken every three minutes for thirty minutes to determine how much water was lost. By dividing the amount of water lost by the leafs surface area, the results of the four conditions can be compared accurately.
The plant placed in the light transpired the most, followed by room temperature, then the fan and mist.
It is evident that water potential greatly impacts transpiration. Since water moves from an area of higher water potential to lower water potential, the drier environments in the lab produced higher rates of transpiration...
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