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Essay Details:
Essay text:
At 39°C 2/4/A1 cells
started to differentiate but simultaneously the cells also underwent massive
cell death.
When cultured at 37°C these cells formed confluent and tight monolayers that
seemed to have paracellular transport characteristics similar to that of the
human intestine. Transmission electron microscopy confirmed the development of
multilayers at 33°C, monolayers at 37°C and defects in the cell layer due to
apoptosis at 39°C.
Different immunostainings of ZO-1, E-cadherin and vinculin confirmed formation
of tight and adherence junctions. Transepithelial resistance reached a plateau
of 25-35 Ohm...
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