Gel Filtration

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Title: Gel Filtration

Essay Details:

Subject: Science

Author: Eunice P

Date: July 26, 2015

Level: University

Grade: B

Length: 2 / 321

Essay text:

This technique can be used particularly in molecular weight determination and on a quantitative analysis of their molecular interactions. “All types of chromatographic methods are based on a very simple principle. The sample to be examined is allowed to interact with two physically distinct entities, a mobile phase and a stationary phase.” (Boyer, 2000) GFC exploits the differences at which molecules travel or pass through a bed of porous, semi-solid substance, which is the gel itself. “The stationary phase consists of inert particles that contain small pores of a controlled size. A solution containing solutes of various molecular sizes are allowed to pass through the column under the influence of controlled solvent flow...

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