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Place the black wild type culture in the “+” quadrants. After these two strains have been inoculated, the mycelia of the 2 strains meet, and perithecia form (1). A rough “X” of black dots, or perithecia should now be visible. Using a toothpick gently scrape the surface of the crossing plate to collect a sample of perithecia. Place the perithecia in a drop of water on a slide, and cover them with a cover slip. Using the eraser of a pencil, press down on the cover slip so that the perithecia break open and release lots of asci with mature ascopores, but be sure not too press down too hard because the ascopores should remain intact in the asci...
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