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Title: - Investigation of the role of MicroRNA- 223, MicroRNA -381 and MicroRNA- 505 in the regulation of transcription factor NFI-A.

Essay Details

Subject: Science
Date: August 21, 2008
Length: 7 / 1981
No of views: 0
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Luciferase reporter assay systems, currently one of the best non-toxic, rapid and sensitive methods used to measure gene expression will be used to determine quantitatively the effect of the selected microRNAs on the expression of NFIA. Constructs consisting of the luciferase gene, NFIA 3’ UTR and different combinations of the miRNAs selected inserted into plasmid vectors and then transfected into cells are expected to have an effect on the amount of luciferase activity measured...

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Results are expected to vary depending on the number and type of miRNA inserted. Additive effects, multiplicative effects and possible interference of the addition of one or more miRNAs will be looked for. Materials and Methods Cell culture and transfection HeLa cells and HEK 293T cells were cultured in DMEM supplemented with 10% FBS...

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