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Essay text:
Both ends compliment each other, which makes the DNA circular once together. This circular DNA is usually present in an infected bacterial cell. This protects the DNA to be degraded by the cell.
In this laboratory experiment, restriction enzymes were used to analyze the DNA of the lambda phage. Restriction enzymes cut DNA in specific locations of the DNA sequence. The restriction enzymes used were EcoR1 and Bam H1. Both of these restriction enzymes cut DNA into six fragments.
Materials
Agarose
Gel Electrophoresis Apparatus
Gel Comb
Gel Mold
100x Triacetate EDTA Buffer with a pH of 8.0
Methylene Blue Stain
Microwave
Micropipette
Ice Bath
Water Bath 37?XC (Incubator)
Water Bath 70?XC (Incubator)
Phage Lambda DNA (uncut)
EcoR1- Restriction Enzyme
EcoR1 + Bam H1 ?V Mixture of Restriction Enzymes
Distilled Water
Methods
?? Preparing the DNA samples
In this lab experiment, four small tubes were labeled 1-4...
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