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The melting point of the yellow, sticky crude product was 91-119 oC. This crude product was then recrystallized from methanol, yielding 0.128g of pale yellow crystals with a melting point of 131-135 oC. This represents 0.33% of the original mass of the two yolks. The cholesterol was then dissolved in ether and further purified by bromination with a bromine/acetic acid reagent and debromination with zinc powder, a series of aqueous washes, and a final recrystallization from methanol. Here, a yield of 28% was recovered from an initial mass of 100 mg of recrystallized material. The melting point of the off-white crystalline final product was 146-148 oC, which is very close to the literature value for cholesterol of 148...
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Common topics in this essay:
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