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Biology Lab

   
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Title: Biology Lab
 
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Subject: Science
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Date: September 25, 2008
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coli in log phase growth 1 vial of plasmid (on ice) Pencil and paper to record data 1 UV light box For Electrophoresis: 1 vial of Lambda DNA cut with HindIII 1 vial of Lambda DNA cut with EcoRI 1 vial of Lambda DNA cut with BamHI 1 vial of Lambda DNA uncut 1 micropippetter (20 ?l) Masking tape 1 gel bed 1 UV light box 1 bottle of Ethidium Bromide 1 bottle of agarose gel 1 practice loading gel 1 power supply 1 comb 1 Electrophoresis chamber 1 bottle of buffer 1 marking pen 1 staining tray Pencil and paper to record data Ruler Method for Collecting Data: 1...
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Mark the sterile 15-ml tubes with “+” and “-“. Use sterile transfer pipette to add 250 ?l ice-cold calcium chloride to the tube. Place the tubes on ice. 2. Use sterile plastic inoculating loop to transfer one or two large colonies of E. coli cells from Starter Plate into your tubes (+ and -)...
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